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With our excellent scientists, Creative Biolabs has accumulated strong expertise and extensive experience in affinity and kinetics measurement for specific antibodies. Creative Biolabs provides Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI) based affinity measurements in a label-free and high-efficiency mode.
Antibody, also known as immunoglobulin, is a Y-shaped protein. Among the five classes of immunoglobulin (including IgA, IgD, IgE, IgG and IgM), IgG plays an outstanding role in immune system. Each individual IgG molecule is unique to target a specific epitope. However, some people have normal levels of immunoglobulins and all forms of IgG, they do not generate sufficient specific IgG antibodies that can protect human from some viruses and bacteria (lack or low level of these IgG molecules). Thus, we call these molecules “specific antibodies”, and they are usually generated to respond to bacteria and viruses, or through the exposure to vaccines. Therefore, specific antibodies are applied extensively in diagnostics.
Those people, who cannot produce specific antibodies in responding to polysaccharide antigens, are diagnosed with specific antibody deficiency (SAD). SAD was fisrt discovered in a group of patients in the early 1980s, and it was one of the commonly identified immune deficiencies among patients presenting severe sinopulmonary infections. Although specific IgG antibodies are important in resisting infections, other immunoglobulin members of our immune system also play vital roles in defensing bacteria and viruses. If these immunoglobulin members work well, the patients with low specific antibodies may rarely be sick. However, antibodies of certain IgG subclasses can interact readily with the complement immune system, immunoglobulin of other subclasses interact poorly with the complement system. In this case, the inability of producing antibodies of a specific subclass can render the individual susceptible to certain kinds of infections. So, it is important to determine the affinity of the specific antibodies.
Creative Biolabs can provide the following affinity measurement for specific antibodies but not limited to:
We can efficiently identify antibody isotypes using Biacore, ProteOn and Octet systems. For instance, when Octect system is applied, the biosensor immobilized with an certain type of anti-species antibody will capture a specific isotype of antibody from a crude sample, such as a hybridoma supernatant. Therefore, identifying the isotype of a specific antibody is achieved.
Figure 1. Diagram of kinetics analysis on Octet system. A biotinylated anti-mouse Fc antibody was immobilized onto Streptavidin biosensors.
A. Saxon et al. (1980). In vitro analysis of humoral immunity in antibody deficiency with normal immunoglobulins. Clin. Immunol. Immunopathol. 17(2): 235-244.
A. Tlili et al. (2006). Impedance spectroscopy and affinity measurement of specific antibody–antigen interaction. Materials Science and Engineering C. 26: 546-550.
P. Estep et al. (2013). High throughput solution-based measurement of antibody-antigen a nity and epitope binning. Landes Bioscience. 5 (2): 270-278.
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