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Ecotin Library Construction

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Creative Biolabs provides the unique ecotin library construction service through our innovative Hi-Affi™ phage display platform. This platform offers the ability to construct high affinity scaffold libraries which can obtain 100% precise mutant and over 1010 diversity.

Ecotin is a group of dimeric serine protease inhibitors found in the periplasm of Escherichia coli and related Gram-negative bacteria that have been shown to be potent inhibitors of many trypsin-fold serine proteases of widely varying substrate specificity. Serine proteases are a large family involved in many important biological processes, such as blood coagulation, fibrinolysis, complement pathways, viral maturation, apoptosis and cancer. In this way, the investigation of natural serine protease inhibitors is an important way to understand the mechanism of serine proteases and develop the inhibitors to become useful therapeutics, especially for the cancer therapy.

As a potent inhibitor of a surprisingly wide range of serine proteases, ecotin is a β-sandwich scaffold designed for serine protease inhibitor selection. All types of ecotins have shown the ability to inhibit pancreatic digestive peptidases trypsin and chymotrypsin, while they can also selectively inhibit several proteases, such as blood peptidases factor Xa, elastase, thrombin and human urokinase-type plasminogen activator (uPA). For instance, uPA is a serine protease that plays an active role in cancer metastasis and tumor invasion, it is considered that the library of phage displayed ecotin variants could be a useful tool for isolating a high affinity inhibitor of uPA, which may contribute to the development of anti-tumor drug and cancer inhibitor. In this way, ecotin is actually possible to play a key role in the treatment of serine protease related diseases.

Hi-Affi platform is developed by Creative Biolabs to combine common phage display technology with the trimer codon technology and NNK method. Compared with traditional phage display technology, which is an exogenous gene expression method through fusing the target genes to bacteriophage coat proteins then displaying on the phage surfaces to select specific binders, Hi-Affi phage display technology shows improved library affinity and diversity. The platform is more suitable for sorting and isolating the high affinity protein or peptide targets.

With extensive experience in the field of scaffold library construction, Creative Biolabs has generated over 50 different kinds of scaffold libraries for our customers all over the world. Our scientists are committed to offer cost-effective service with the highest quality to assist our clients’ research project constantly moving forward.

Ecotin Library Construction Fig. 1 Ribbon plot of a 2.0 Å structure of mEcotin, showing β-sheets in yellow, helices in magenta, and flexible loops and turns in blue. The introduced β-turn is labeled red. (Eggers et al. 2001)

Ecotin Library Construction Fig. 2 Crystal structure of a monomeric form of general protease inhibitor, ecotin in absence of a protease. (PDB ID: 1IFG)

Reference:
Eggers, C. T., Wang, S. X., Fletterick, R. J. and Craik, C. S. (2001) 'The role of ecotin dimerization in protease inhibition', Journal of molecular biology, 308(5), 975-991.




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