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GCN4 Library Construction

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Creative Biolabs provides the best GCN4 library construction service with high quality, diversity and affinity to fulfill every customer’s individual requirements. Besides GCN4 library, our scientists have built 55 kinds of scaffold libraries for our clients throughout the world, such as DARPin, charybdotoxin and EETI-II.

The protein GCN4, belongs to the basic leucine zipper (bZIP) family, is isolated from yeast Saccharomyces cerevisiae. GCN4 is known as a transcription factor responsible for both the activation of more than 30 genes required for amino acid or purine biosynthesis and the response to amino acid or purine starvation. The structure of the GCN4 is relatively simple and the determinants for stability are well characterized. It consists of two 34 amino acid α-helices that form a stable, homodimeric coiled coil, which is a structural motif in proteins in which 2-7 α-helices are coiled together like the strands of a rope. Furthermore, the GCN4 leucine zipper, with a midpoint transition temperature of 57°C, carries the only moderately stable coiled coil. When bound to the target, the GCN4 coiled coil can potentially adjust its helical structure to effectively present binding epitopes on the surface. In this way, it allows various modification to maximize the amount of replaced residues on one hand, and on the other hand minimize disturbance to the scaffold structure while designing the protein grafting. Combining with phage display technology, Creative Biolabs is able to develop GCN4 scaffold libraries for further investigation of this transcription factor.

Phage display technology has become a major approach in selecting highly specific scaffolds for human therapy. It is an exogenous gene expression method through fusing the target genes to bacteriophage coat proteins and then displaying on the phage surface to select specific binders. Differ from other companies, the scaffold libraries of Creative Biolabs are generated through our proprietary Hi-Affi™ phage display platform, which has improved the diversity and affinity of specific protein scaffolds by using trimer codon technology and NNK method. This technology ensures our scaffold libraries to achieve 100% precise mutant and over 1010 diversity.

With extensive experience in the field of scaffold library construction, Creative Biolabs always provide the best services for our customers. The timely quotation, high quality service, fast delivery cycle and the most competitive price are our commitment.

GCN4 Library Construction Fig. 1 GCN4-leucine zipper core mutant ASN16GLN in the trimeric state. (PDB ID: 1ZIM)




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