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Creative Biolabs offers a novel in vivo phage display method for clients all over the world. This method can be used for specifically and efficiently isolating the tissue-specific peptides bound to cell markers or cell-penetrating peptides (CPPs) for transporting chemotherapeutic agents. Our technical scientists are aimed to provide a reliable and robust selection system to meet our customer’s individual requirements.
In vivo phage display is a unique high-throughput method for biopanning by which the peptide phage libraries are directly injected into animals for isolating and identifying the phages bound to specific tissues. Compared with other panning methods, the targets used for isolating phage-displayed peptides are more “intact” with the natural molecular structure and are in the functional states in vivo. Those selected peptides can be used as selective protein transduction domains (PTD) or cell-penetrating peptides (CPPs) for transporting chemotherapeutic agents to the specific tissue, thus improving drug specificity and efficacy in reducing toxicity. Furthermore, some selected peptides can block the function of their ligand proteins by themselves. Using this method, we can offer the functional analysis of new receptors and potential identification and development of novel drug target candidates for the customers.
Our services of identifying targeting peptides for different tissues/organs by in vivo phage display including but not limited to:
Creative Biolabs has adopted this novel in vivo phage display technology to develop a specific platform for selecting tissue-specific peptides. Through this platform, our scientists can generate high diversity phage display libraries with more than 109 different clones. The libraries will then be used to broadly interrogate the surface of various cells and specific isolate protein ligands from target tissues. The selection results may display homology to known proteins which can be developed as diagnosis, or uncharacterized proteins which represent potential novel biomarkers.
Creative Biolabs has years of experience in the field of in vivo phage display. Our scientists are professional in isolating highly specific binders for various targets and confident in providing clients with the best service at the most competitive cost.
Fig. 1 Schematic representation of the in vivo bio-panning for homing peptide/receptor pairs. (Zhang et al. 2005)
Zhang, L., Hoffman, J. A. and Ruoslahti, E. (2005) 'Molecular profiling of heart endothelial cells', Circulation, 112(11), 1601-1611.
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