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In vivo Phage Display for Muscle Targeting

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Creative Biolabs offers clients a powerful service for isolating muscle targeting peptides by in vivo phage display method. These muscle targeting peptides have many potential applications for oligonucleotide or drug delivery to muscle for myopathies. Besides that, this method identifies muscle cell-surface receptors that can be used as potential targets for genetic modification of adenovirus tropism.

Muscle, as an indispensable part of almost all animal species, makes up largest tissue volume of the body, while also yields several challenges and difficulties for muscle-specific therapy. Normal life activities, such as respiration, ambulation and cardiac function, are mainly related to a series of muscles including skeletal, cardiac and laryngeal muscles. However, a large group of inherited or acquired myopathies are existing worldwide and affecting the ability of muscles to exert their function for both adults and children. At present, gene therapy shows particularly attraction for the treatment of various inherited myopathies, such as muscular dystrophies and heart failure. Ideally, the therapy should be carried out through specifically targeted vector construct or pharmaceuticals to muscles following intravenous injection. But up to now the targeting mechanisms for mature skeletal or cardiac muscle are still absence. Thus, the identification of muscle targeting moieties (e.g. muscle-specific peptides) is meaningful and valuable. One particular choice is applying in vivo phage display, which can provide high specificity and diversity candidates to reduce the oligonucleotide dosage of therapy or minimize the possibility of non-muscle toxicity.

Innovative scientists of Creative Biolabs have developed a novel in vivo phage display platform for selecting tissue-specific peptides. Using this method, a great deal of peptides or protein ligands candidates, which is specific for the target muscle tissues, can be isolated form cell surface. Those selected peptides will contain fully in vivo characteristics and can be used for transporting therapeutic agents to muscles and improve drug specificity and efficacy. In addition, it is also possible to identify some novel muscle cell-surface receptors by this method and then provides important tools for improving the efficiency of gene transfer in mammalian muscles, such as developing targeted adenoviral vectors.

Creative Biolabs has been a long-term expert in the field of phage display. Our most devoted scientists are confident in offering our customers the best service to meet all individual required specifications.

In vivo Phage Display for Muscle Targeting Fig. 1 Schematic representation of isolating muscle targeting peptides by in vivo phage display method. (Seow et al. 2010)

Reference
Seow, Y., Yin, H. and Wood, M. J. (2010) 'Identification of a novel muscle targeting peptide in mdx mice', Peptides, 31(10), 1873-1877.




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