Creative Biolabs develops and commercializes a full range of integrated innovative services that are based on phage display technology. We have ...
Creative Biolabs can offer advanced protein engineering platform for your specific project, including high-scale expression, crystallization and characterization...
Creative Biolabs has established custom membrane protein and membrane protein antibody production platforms for antibody discovery...
Creative Biolabs provides a full range of services based on our matured hybridoma platform. Our featured services involve the custom monoclonal antibody production, from ...
Given the importance of membrane protein targets (multi-spanner), private and academic laboratories have tried numerous approaches to raise antibodies against them. The most common way of generating these antibodies is immunization with whole cells over-expressing the target protein. This allows membrane protein targets to be displayed in their native conformation without mechanical or detergent disruption. Typically, stable murine cells expressing a human membrane protein are used to immunize mice. Using this approach, a number of antibodies against highly expressing membrane proteins have been developed. Transiently-transfected cells, membrane preparations, and cells selected for the highest expression levels have been used to improve this approach, with varying degrees of success. However, for many important membrane protein targets the ability of cell-based immunogens to elicit high-quality antibodies has been limited by low membrane-protein expression, the abundance of non-specific proteins, and target protein toxicity during cell line selection.
The other classic approach is to design peptide antigens derived from the extracellular loops for antibody production. Unfortunately, these peptide antigens could not fold in correct tertiary structures as within multi-spanner, thus they are not good for making conformational antibody against multi-spanners.
The overcome this hurdle, we have developed MPAT™ platform (MPAT=Membrane Protein Antibody Technology)
Reconstituted Protein in
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