Follow us on:
Dedication / Services
To provide 50000 services and products to global scientific research units every year

Peer-Reviewed Publications

Bookmark and Share

Creative Biolabs has provided high-quality antibody discovery, engineering and manufacturing services to numerous customers. A long list of papers has been published in peer-reviewed journals and a lot of patents have been approved or in process which cited custom products or services from Creative Biolabs. Below are some selected patents and articles to give you an idea of how we assist life science research in academia and industry.

For your convenience, we have grouped these patents and articles into the following categories:



SECTION 1: Phage Display Services and Products[Top]

Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages to connect proteins with the genetic information that encodes them. Creative Biolabs is a leading service provider that focuses on phage display technology. Our services include phage display library construction and screening.


The paper describes Creative Biolabs’ scientists panned SPARC against the premade human Fab phage display library HuFabL of Creative Biolabs for the client.


The paper describes Creative Biolabs’ scientists isolated mouse anti-C1Q binding site and mouse anti-FcgR1 binding site Fabs from a phage display library made in Creative Biolabs’ Lab, and expressed them as soluble proteins.


The paper describes Creative Biolabs is a custom service company from where you can get naïve human Fab phage library generated as client demand.


The patent describes the client used a premade phage display libraries having synthetic peptides from Creative Biolabs and accomplished their study.


The patent describes Creative Biolabs supplied a M13 bacteriophage capable of binding human CTLA for the client.


The paper describes Creative Biolabs accepts service contract from any commercial premade library, a custom-constructed one in the company, or a hand-made one.


The paper describes the cDNA library was synthesized using the SMART™ cDNA Library Construction Kit at Creative Biolabs and a total of 2,462 ESTs were 5' sequenced in Creative Biolabs.


The paper describes the author used custom-made phage libraries from Creative Biolabs for material science applications.


The paper describes Creative Biolabs is a company focused on the development of VH- and/or VL-based therapeutics and affinity reagents.


This book describes Creative Biolabs is custom phage display library supplier for immunized, or naïve, and semisynthetic/synthetic antibody libraries, phage display peptide libraries, cDNA phage display libraries.


The author’s work used Creative Biolabs’ Human Single Domain Antibody Library (HuSdL®), which was constructed, based on the camelized human VH3 with elongated and randomized HCDR3 sequences, and has a calculated diversity of 1.5 × 10 9 as per the manufacturer.


Creative Biolabs provided several types of HuScL libraries for the author.


Creative Biolabs provided the helper phage KM13 (1.0 × 1011 pfu/mL) for the author.


This author mentioned that the most widely used commercial phage display libraries are from Creative Biolabs, which contains 10 7 -10 variants.


Creative Biolabs constructed a Single-chain Variable Fragment Antibody Library against Fusobacterium Nucleatum for the author.


scFvs used in these patents were selected from a fully human scFv library displayed on bacteriophage, from Creative Biolabs, and the scFv phage selection was conducted under contract with Creative Biolabs.


Creative Biolabs used antibody phage display libraries to complete the antibody populations for ES-ICER expression.


Creative Biolabs provided the authors the HuSdL® phage display human single domain antibody library.

SECTION 2: Hybridoma Services [Top]

Creative Biolabs provides a full range of services in custom polyclonal and monoclonal antibody production from gene expression or peptide synthesis to antibody purification and labeling. We have the capacity of producing antibodies in either research quantities or in large scale for industrial applications. We do epitope prediction, peptide synthesize, protein expression, polyclonal or monoclonal antibody production as well as antibody purification from cell culture, ascites fluid or whole serum. We can also generate mono-specific polyclonal antibodies by ligand affinity chromatography.


The paper describes that five BALB/C mice were immunized with the full-length human recombinant FGF23 using a Bac-to-Bac baculovirus expression system and the fusion cells showing positive reaction to FGF23 were screened with the Omni Hybridoma Platform and cloned in Creative Biolabs.


The paper describes a study from which a hybridoma cell line was cultured and validated in Creative Biolabs' Lab. Creative Biolabs’ scientists synthesized first-strand cDNA, then constructed the Fab and scFv with phagemid pDisplay-4™ and pDisplay-2™ respectively, and expressed the soluble Fab and scFv exactly as the study designed.


The paper describes that Creative Biolabs’ scientist developed anti-human Duox mAb, S-12, using hybridoma technique.


The paper describes the mouse monoclonal antibody specific for PKD1 was raised by Creative Biolabs against a 21-amino acid peptide in the N-terminal of human PKD1, which is not present in PKD2 and PKD3.

The paper describes Creative Biolabs generated the antibodies specific for three peptides from the ORF of TDF protein and used in both IHC and WB to confirm anti-TDF Ab results.


The paper describes expression of human Nox5, immunization of mice and Nox5 monoclonal antibody production by hybridoma technique were carried out by Creative Biolabs. Finally, 8 positive clones were selected and evaluated further. Isotyping of 8 clones identified 7 of them as IgG clones.


The paper describes the generation of FAM20C monoclonal antibodies with the Omni-Hybridoma platform from Creative Biolabs. Three positive clones (clones 3, 5, and 6) with the IgG isotype were further screened using IHC and Western immunoblotting analyses.


The paper describes anti-VLPs mAb production was carried out at Creative Biolabs using hybridoma technique.


The paper describes mouse anti-human Duox2 monoclonal antibody S-40 was developed by Creative Biolabs by using hybridoma technique.


The paper describes to precisely determine the expression status of SAG in human tumor tissues, scientists of Creative Biolabs raised an antibody against purified human SAG RING domain (AA44-113) fused with glutathione S-transferase, and performed immunostaining analysis using the SAG mAb specificity against SAG.


The paper describes a mouse anti-iNOS monoclonal antibody created at Creative Biolabs used to analysis the CXCL10 and CXCL9 expression by immunocytochemistry.


The paper describes anti-TDF antibodies were custom-made by Creative Biolabs using TDF peptide P1 (TDF-P1) as an immunogen. Creative Biolabs’ scientists synthesized TDF-P1 peptide and coupled them to KLH, then used for immunization of rabbits. The correct sequence of TDF-P1 was confirmed by MS and the specificity of the antibody was confirmed by pre-incubation of anti-TDF antibodies with its peptide antigen in an inhibition assay.

SECTION 3: Antibody Engineering and Antibody Sequencing[Top]

Creative Biolabs is a recognized service provider in converting small gene-engineered scFv or Fab human/mouse antibodies derived from phage display antibody library screening or mouse/rat hybridoma cell lines into full-size recombinant human or mouse IgGs with Fc fragments of various functions, e.g. ADCC, or non-ADCC. Creative Biolabs has established a solid platform for industrial scale production of recombinant antibodies in various expression systems.

Creative Biolabs has developed the proprietary Database Assisted Shotgun Sequencing (DASS) technology, which based on our next generation antibody sequencing platform. Purified monoclonal antibodies in multivalent forms can be sequenced with 100% coverage and 100% accuracy.


The paper describes that the heavy and light chain variable regions of a mouse monoclonal antibody (mAb 5.91) with high affinity binding to an epitope in the C2 domain of the epsilon chain of canine IgE were sequenced by Creative Biolabs.


The patent suggests Creative Biolabs can design and produce fully humanized antibody.


The paper describes Creative Biolabs found the sequence errors in the variable heavy chain and fixed them, then produced the IgG protein.


The paper describes Creative Biolabs expressed and purified a mutated Humira IgG from HEK 293E cells. We incorporated a cysteine mutation in place of Ser 258 in each heavy chain exactly as the author demanded.


The paper describes the heavy and light chain antibody F(ab) fragments were cloned, sequenced and inserted in tandem into an expression vector, expressed in HEK 293 cells with a similar binding features to that of the parent hybridoma.


The paper mentioned that Creative Biolabs can reshape non-human antibody into other non-human antibodies such as antibody caninization.


Creative Biolabs serviced the papain digested humanized recombinant trastuzumab (2 mg/mL) for the author.


Creative Biolabs expressed the humanized IgG antibody for the author, and fixed the sequence errors found in the variable heavy chain for the author.


Creative Biolabs expressed the mutated Humira IgG (hIgG), in HEK 293E cells and purified using a HiTrap rProtein A FF column for the authors.


Creative Biolabs amplified and sequenced the complete cDNA sequence of Hybridoma cell line 11D8 for the authors, then produced and purified the recombinant antibodies.


The paper describes a cell-permeable peptide containing the hydrophobic region of the signal peptide of Kaposi fibroblast growth factor (AAVALLPAVLLALLAP)15 and a single NLS from NF-kB (VQRKRQKLMP)16 was synthesized and cyclized (intrachain disulfide bond between cysteines inserted in the NLS motif) by Creative Biolabs.


The paper describes the genome sequencing, including library preparation, Solexa cluster generation and single-end-read sequencing, was performed by Creative Biolabs.

SECTION 4: Single Domain Antibody Services[Top]

Creative Biolabs is specialized in generating single domain antibodies against any targets. We also provide a wide range of services, including construction of immunized single domain antibody libraries using llama and camel, construction of synthetic camelized human single domain antibody libraries using DNA synthesis, biopanning of single domain antibodies libraries and large scale production of recombinant single domain antibodies. Together with Creative Diagnostics, we provide a large list of single domain antibodies.


Creative Biolabs provides customized services of single domain antibodies.


The cells were grown to 50% to 70% confluence, then disrupted in freshly prepared ice-cold lysis buffer as described above for 45 minutes on ice. Lysates were then centrifuged, the supernatant collected, and protein concentration was determined as described above. Total protein (1 mg) was incubated with 1.5 mg of Dynabeads (Invitrogen) containing the bacterial anti-STAT3 VHH13 (SEQ ID NO:3) or negative control (KRAS, Creative Biolabs, Shirley, NY) for 1 hour at 4°C.

SECTION 5: Yeast Two-Hybrid[Top]

Creative Biolabs provides the services on a strict fee-for-service basis. We are professional in using Gal4 and LexA based Y2H systems. We are able to perform yeast two-hybrid screening against integral membrane proteins and membrane-associated/proximal proteins using a modified split-ubiquitin membrane yeast two-hybrid (MYTH) system.


The paper describes Creative Biolabs performed a yeast two-hybrid screen and assay against an adult human brain cDNA library.

SECTION 6: Antibody Development in Various Animal Species[Top]

Creative Biolabs provides antibody production service using a comprehensive list of host species.


The paper describes anti-TDF antibodies were custom-made by Creative Biolabs using TDF peptide P1 (TDF-P1) as an immunogen. Creative Biolabs’ scientists synthesized TDF-P1 peptide and coupled them to KLH, then used for immunization of rabbits. The correct sequence of TDF-P1 was confirmed by MS and the specificity of the antibody was confirmed by pre-incubation of anti-TDF antibodies with its peptide antigen in an inhibition assay.


The paper describes the inducible nitric oxide synthase (iNOS) antibody used in IHC was purchased from Creative Biolabs.


The paper describes the author used a stock solution containing 14 μM IgY from a chicken immunized with ADP3 from Creative Biolabs.


Creative Biolabs produced rabbit antiserum for mouse PERK phosphorylated at T779 by immunizing animals against a chemically synthesized phosphopeptide of mouse PERK conjugated with keyhole limpet hemocyanin (KLH).


Creative Biolabs produced anti-ADP3 chicken IgY by immunizing a chicken with ADP3.


The mice were fed an atherogenic diet containing 1.25% cholesterol and 21% milkfat for 5 months to increase their LDL level and were used to isolate LDL which was afterwards oxidized and adequately characterized before being sent to Creative Biolabs where it was used to immunize Balb/c mice.


Creative Biolabs generated two rabbit polyclonal anti-TDF antibodies: anti-TDF-P1 antibody (anti-TDF-P1-Ab) and anti-TDF-P1P2P3 antibody (anti-TDF-P1-Ab) for the author.


The paper describes TDF-P1 peptide, with the amino acid sequence H2-RESQGTRVGQALSFLCKGTA-COOH, was synthesized, and rabbit poyclonal TDF generated against TDF-P1 by Creative Biolabs.

SECTION 7: Fluorescent In Situ Hybridization (FISH)[Top]

Creative Biolabs offers a full array of custom fluorescence in situ hybridization (FISH) service from probe design, chromosome/cell preparation to expert result interpretation.


The paper describes Creative Biolabs performed FISH on a normal primary neonatal dermal fibroblast cell line of European origin (PCS-201-010 from ATCC® Primary Cell Solutions™) following standard procedures with short probe length (~1 kb).


This author mentioned that Fluorescent in Situ Hybridization (FISH) was performed according to the instructions of Creative Biolabs.

SECTION 8: In Situ Hybridization (ISH)[Top]

Creative Biolabs offers a full array of custom in situ hybridization (ISH) service from probe design, tissue acquisition to expert interpretation of gene expression results.


The paper describes the commercially available prostate tissue microarrays (TMAs) used in this study were purchased from Creative Biolabs.


The paper describes IHC for myeloid-associated differentiation marker was carried out using tissue microarrays from Creative Biolabs.


The paper describes the study used formalin fixed, paraffin embedded tissue, liver tissue sections and liver cancer tissue microarrays were obtained from Creative Biolabs.


The paper describes the human glioma tissue arrays were purchased from Creative Biolabs.


The paper describes Creative Biolabs can not only offer microarray product but also help the client in analyzing of microarray data.


The paper describes the study used the fibronectin-coated coverslips from Creative Biolabs.


The paper describes the formalin-fixed and paraffin-embedded tumor specimens used in this study were from Creative Biolabs.


The paper describes a breast carcinoma progression tissue microarray used for immunohistochemical staining for Nodal was supplied by Creative Biolabs.


The paper describes the normal human lung and 67 different lung tumors were purchased from Creative Biolabs.


The paper describes the normal human lung and 67 different lung tumors were purchased from Creative Biolabs.

SECTION 9: Immunology Experimental Technique[Top]

The patent describes Creative Biolabs’ scientists performed the immunohistochemical staining for Nodal in a breast carcinoma progression TMA for the client.


The patent describes Creative Biolabs served Formalin-fixed and paraffin-embedded tumor specimens for them used in this study.

SECTION 10: Recombinant Protein Products[Top]

Creative Biolabs offers extensive expertise across four expression systems for producing your recombinant proteins, monoclonal antibodies, vaccine antigens or disease biomarkers. Whether for structural studies, functional assays, target validation or high throughput screens, Creative Biolabs can give a hand. We can supply proteins expressed by the following systems: Bacterial expression systems (E. coli / Bacillus); Yeast expression systems (P. pastoris / S. cerevisiae); Baculovirus-insect cell expression systems; Mammalian expression systems (CHO / 293); membrane protein production (New).


Uniformly 13C- and 15N-labelled CaM was purchased from Creative Biolabs.


The patent study used recombinant human EGF (hEGF) from Creative Biolabs.


The patent study used ADP3 from Creative Biolabs.


The paper describes nearly all human recombinant cytokines they used in the study are purchased from Creative Biolabs.


The paper describes iNOS was supplied by Creative Biolabs.


The paper describes the purified recombinant WT mouse CPE was custom generated by Creative Biolabs.


The paper describes mouse CPE the author used in the study was expressed and purified by Creative Biolabs.


The paper describes the author used two types of TMAs including one breast cancer progression TMA that was purchased from Creative Biolabs.


The paper describes the recombinant human EGF used in this study is from Creative Biolabs.


The paper describes recombinant mouse full length CPE custom expressed in HEK293 cells and purified by Creative Biolabs.


Creative Biolabs produced the recombinant mutated onchocystatin in Escherichia coli.


The paper describes YeastXceed™ Technology is available from Creative Biolabs.


Our customer service representatives are available 24 hours a day, from Monday to Sunday. Contact Us

Online Inquiry
Name:
Phone:
*E-mail Address:
*Service & Products Interested:
Project Description:
*Verification Code:
Please input "biolabs"(case insensitive) as verification code.
Contact Us

USA
Tel: 1-631-871-5806
Fax: 1-631-207-8356
Email:

Europe
Tel: 44-207-048-3343


© 2007 - 2016 Creative-Biolabs All Rights Reserved