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Phosphorylation-Specific Antibody Production Services

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Phosphorylation events are commonly probed by phosphorylation-specific antibodies. With years of in-depth research in phosphorylation binding domains and application of High-Affi™ technology, Creative Biolabs can produce high-affinity antibodies that recognize this most widely studied post-translational modification, phosphorylation.

Phosphorylation is a ubiquitous and essential post-translational modification (PTM) by covalent addition of a phosphate (PO43−) group to a protein or other organic molecule. Protein phosphorylation is mediated by kinases and phosphatases. This reversible phosphorylation and dephosphorylation action affects an estimated one-third of all proteins and involves many critical events in cellular response, such as cellular localization, protein degradation, protein conformational change, signal transduction, enzymatic activity regulation, and protein-protein interaction. Abnormal phosphorylation can cause a range of diseases, such as neurodegenerative diseases, cancer, diabetes, cardiovascular diseases, and autoimmune diseases. On a given protein, phosphorylation usually occurs on many distinct sites and respective amino acids catalyzed by kinds of kinases. In eukaryotic proteins, it occurs on serine, threonine, tyrosine or histidine residues. While in prokaryotic proteins, it occurs on serine, threonine, tyrosine, histidine, arginine or lysine residues. Serine is the most common phosphorylation site, followed by threonine. Particularly, tyrosine phosphorylation is relatively rare but is a key step at the origin of protein phosphorylation signaling pathways in most parts of the eukaryotes.

Phosphorylation-Specific Antibody Production Services Fig. 1 Strategy for generating phospho-specific antibodies.

Creative Biolabs offers phospho-pan/phospho-site specific antibodies against phosphorylated amino acids listed below:

phospho-Serine phospho-Threonine
phospho-Tyrosine phospho-Histidine
phospho-Arginine phospho-Lysine

With the time-saving High-Affi™ technology developed by Creative Biolabs, we can customize antibodies with high specificity and affinity for phosphorylation protein detection. These antibodies are suitable for many research applications, including Western blot analysis, ELISA, immunoprecipitation, and immunocytochemical assays. They are also available for investigative and diagnostic pathology as hundreds of phosphorylation state-specific antibodies make possible the study of protein phosphorylation in situ.

Custom Phosphorylation-specific Polyclonal Antibody Production

Based on years of professional antibody production services, Creative Biolabs has established phospho-specific polyclonal antibody production services for customers. Following phospho-specific peptide synthesis, KLH carrier conjugation, immunization, and antisera production, all antibodies are purified by using a double affinity column chromatography of nonphospho-peptide and phospho-peptide. The monophospho-specific antibody is then tailored to your needs after being fully characterized. We also offer the manufacture of dual phospho-specific antibodies which is suitable for the detection of two residues that are both phosphorylated and close enough (5 amino acid residues) within the same epitope.

In addition to the phosphorylation-specific antibody, Creative Biolabs also provides a comprehensive list of PTM-specific antibody production services of your choice.

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