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Primers used in the construction

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Primers from Barbas III's book [Reference 1]
(M : A or C; R: A or G; S: G or C; Y :5 C or T).
Kb1, 5'-GGGCCCAGGCGGCCGAGCTCGTGMTGACCCAGACTCCA-3'
Kb 2, 5'-GGGCCCAGGCGGCCGAGCTCGATMTGACCCAGACTCCA-3'
Kb 3, 5'-GGGCCCAGGCGGCCGAGCTCGTGATGACCCAGACTGAA-3'
kf1, 5'-aga tgg tgc agc cac agt tcg tAGGATCTCCAGCTCGGTCCC-3'
kf 2, 5'-agatggtgcagccacagttcgtTTGATTTCCACATTGGTGCC-3'
kf 3, 5'-agatggtgcagccacagttcgtTTGACSACCACCTCGGTCCC-3'
lb,5'-GGGCCCAGGCGGCCGAGCTCGTGCTGACTCAGTCGCCCTC-3'
lf,5'-agatggtgcagccacagttcgGCCTGTGACGGTCAGCTGGGTCCC-3'
hb1, 5'-GCTGCCCAACCAGCCATGGCCCAGTCGGTGGAGGAGTCCRGG-3'
hb2, 5'-GCTGCCCAACCAGCCATGGCCCAGTCGGTGAAGGAGTCCGAG-3'
hb3, 5'-GCTGCCCAACCAGCCATGGCCCAGTCGYTGGAGGAGTCCGGG-3'
hb4, 5'-GCTGCCCAACCAGCCATGGCCCAGSAGCAGCTGRTGGAGTCCGG-3'
hb1-n, 5'-GCTGCCCAACCAGCCATGGCCCAGTCGGTGGAGGAGTCC-3'
hb2-n, 5'-GCTGCCCAACCAGCCATGGCCCAGTCGGTGAAGGAGTCC-3'
hb3-n, 5'-GCTGCCCAACCAGCCATGGCCCAGTCGYTGGAGGAGTCC-3'
hb4-1-n, 5'-GCTGCCCAACCAGCCATGGCCCAGCAGCAGCTGRTGGAGTCC-3'
hb4-2-n, 5'-GCTGCCCAACCAGCCATGGCCCAGGAGCAGCTGRTGGAGTCC-3'
hf,5'-CGATGGGCCCTTGGTGGAGGCTGARGAGAYGGTGACCAGGGTGCC-3'
HKC-F, 5'-cgaACTGTGGCTGCACCATCTGTC-3'
lead-B, 5'gGCCATGGCTGGTTGGGCAGC-3'
lead-B-n, 5'TGgGCCATGGCTGGTTGGGCAGC-3'
HIgGCH1-F, 5'GCCTCCACCAAGGGCCCATCGGTC-3'
Dpseq, 5'-AGAAGCGTAGTCCGGAACGTC-3'
RSC-F, 5'-GAGGAGGAGGAGGAGGAGGCGGGGCCCAGGCGGCCGAGCTC-3'
lead-VH, 5'-GCTGCCCAACCAGCCATGGCC-3'
dp-EX (5'-GAGGAGGAGGAGGAGGAGAGAAGCGTAGTCCGGAACGTC-3',
ompseq, 5'-AAGACAGCTATCGCGATTGCAG-3'
pelseq, 5'-CTATTGCCTACGGCAGCCGCTG-3'


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