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Recombinant Human Epidermal Growth Factor

DESCRIPTION CATALOG # SIZE PRICE
Recombinant Human Epidermal Growth Factor CRP0819 1mg Please Inquiry
Product Detail
Cat
CRP0819
Product Overview
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
Specificity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 2 ng/ml, corresponding to a specific activity of 1.0 x 105 IU/ mg.
Endotoxin
Less than 0.3ng/ug (0.3IEU/ug) determined by LAL test.
Purity
>95% as determined by SDS-PAGE and SEC-HPLC.
Storage
Lyophilized rHuEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution rHuEGF should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended to reconstitute the lyophilized rHuEGF in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Recombinant Human Epidermal Growth Factor
PDB rendering based on 1ivo.
Background
Antigen Description
Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. EGF is a small 53 amino acid residue long protein that contains three disulfide briges.
Function
calcium ion binding; epidermal growth factor receptor activating ligand activity; growth factor activity; protein binding
Gene ID
1950
Synonyms
HOMG4, URG, Urogastrone, Pro-epidermal growth factor;Pro-epidermal growth factor precursor; epidermal growth factor (beta-urogastrone)
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