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Recombinant Human Pegylated Granulocyte-Colony Stimulating Factor

DESCRIPTION CATALOG # SIZE PRICE
Recombinant Human Pegylated Granulocyte-Colony Stimulating Factor CRP0809 1mg Please Inquiry
Product Detail
Cat
CRP0809
Product Overview
Recombinant Human G-CSF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Dalton. PEG-GCSF is manufactured by attaching a 20,000 Dalton methoxypolyethylene glycol propionaldehyde (mPEG-ALD) to the N-terminal amino acid of G-CSF having a total molecular mass of 38800 Dalton.
Specificity
PEG-GCSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 1.0 x 107 IU/mg.
Endotoxin
Less than 0.1 ng/ug (1IEU/ug) of PEG-GCSF.
Purity
>95% as determined by HPLC, FPLC and SDS-PAGE Silver Stained gel.
Storage
PEG-GCSF although stable at 15oC for 1 week, should be stored between 2oC -8oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended to reconstitute the lyophilized PEG-GCSF in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Recombinant Human Pegylated Granulocyte-Colony Stimulating Factor
Crystal structure of 3 molecules of human G-CSF. FromPDB1rhg
Background
Antigen Description
A glycoprotein of MW 20 kDa containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by IL-17.
Function
cytokine activity; enzyme binding; granulocyte colony-stimulating factor receptor binding; interleukin-6 receptor binding.
Gene ID
1440
Synonyms
G-CSF; GCSF; colony stimulating factor 3 (granulocyte); colony stimulating factor 3; granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; Granulocyte colony-stimulating factor precursor
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