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Recombinant Human Stem Cell Factor

DESCRIPTION CATALOG # SIZE PRICE
Recombinant Human Stem Cell Factor CRP0827 1mg Please Inquiry
Product Detail
Cat
CRP0827
Product Overview
Recombinant Human SCF produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
Specificity
The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is less than 2 ng/ml, corresponding to a Specific Activity of 5.0 x 105 IU/ mg.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of rHuSCF.
Purity
>95% as determined by SEC-HPLC and SDS-PAGE.
Storage
Lyophilized rHuSCF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution rHuSCF should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.
Reconstitution
It is recommended to reconstitute the lyophilized rHuSCF in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Recombinant Human Stem Cell Factor
PDB rendering based on 1exz.
Background
Antigen Description
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Function
growth factor activity; identical protein binding; stem cell factor receptor binding
Gene ID
4254
Synonyms
KL-1, Kitl, MGF, SCF, SF, SHEP7, C-kit ligand, Stem cell factor, Mast cell growth factor, Kit ligand; Kit ligand Precursor
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