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1) Amplification of rabbit VK and VL

Template: rabbit blood, spleen or bone marrow cDNA

Amplification of rabbit VK and  VL

2) Amplification of rabbit VH

Template: rabbit blood, spleen or bone marrow cDNA

Template: rabbit blood, spleen or bone marrow cDNA

3) Amplification of CK

Template: pCDisplay-4 phagemid DNA

Template: pCDisplay-4 phagemid DNA

4) Amplification of CH1

Template: pCDisplay-4 phagemid DNA

Template: pCDisplay-4 phagemid DNA

5) Assembling of light chain

Assembling of light chain

6) Assembling of heavy chain

Assembling of heavy chain

7) Assembling of chimeric Fab

Assembling of chimeric Fab


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