With substantial experience in T cell receptor (TCR) engineering and TCR transfer gene therapy, Creative Biolabs provides a broad range of assay services to evaluate the antigen specific reactivity and cytotoxicity of TCR-modified T lymphocytes. Taking advantage of state-of-the-art instruments and experienced Ph.D level TCR specialists, we are capable of designing and performing custom analysis service to obtain comprehensive information about the reactivity and cytotoxity of your specific TCRs.
Recently, an innovative immunotherapy approach involving substituting redirected T cells using predefined certain antigen-specific TCR gene transfer has shown great promise in treating multiple caners and infection diseases. Gene modified TCR therapy is employing certain delivery vehicle to transfer the α-and β-chains genes of a specific TCR into autologous or donor-derived T cells, as a result, the specificity of those T cells can be redirected and large populations of antigen-specific T cells can be generated with high avidity for the antigen of interest. A successful engineered T cell with antigen–specific TCR can harness their own cytotoxicity to the target and eradicate tumors or other diseased cells, therefore, it is critical to analyze the lymphocyte antigen reactivity and cytotoxicity (target-killing activity) with appropriate approaches, which is also an approach to evaluate the effiency of your TCR. Creative Biolabs has various assay methods available for customers to measure the antigen specificity and cytotoxicity of TCR-modified T cells. According to the fact that the activation of T lymphocytes, both in vivo and in vitro, induces expression of CD69, we strongly recommended CD69 induction assay based on the flow cytometric analysis of CD69 expression to analyze the antigen-specificity of the cloned TCR α/β pairs. Additionally, the specificity and biologic activity of TCR gene-transduced T-cells can be determined by cytokine production, using enzyme-linked immunosorbent assay (ELISA) for the detection of cytokine secretion can be an alternative approach. The ability of the transduced cells to lyse target cancer cells (cytotoxicity) can be measured by the chromium release assay and the calcein-AM release assay. Other methods suitable for the analysis of antigen specificity and cytotoxicity are also available upon request. Meanwhile, Creative Biolabs is willing to custom a one-stop evaluation procedure according to your requirement.
Customers choosing our lymphocyte antigen reactivity and cytotoxicity assay service of TCR gene-transduced T-cells will benefit from our advanced instruments and extensive expertise. We are dedicated to offer top-quality service to meet every customer’s requirements.
High Efficiency TCR Gene Transfer into Primary Human Lymphocytes Affords Avid Recognition of Melanoma Tumor Antigen Glycoprotein 100 and Does Not Alter the Recognition of Autologous Melanoma Antigens.2003. The Journal of Immunology.
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