pCDCAR1 C2 m(28ζ), T(CAR-T-2-M304-2Z)

The vector of anti-C2γ and C2ζ chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target mouse C2γ and C2ζ. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-C2γ and C2ζ antibody linked to CD28 transmembrane domain/ endodomain and CD3-zeta signaling domains. And the vector product was designed for the treatment of autoimmune arthritis.


Details


Target
C2
Target Species
Mouse
Targeting Cell Type
T cell
Targeting Diseases
Autoimmune arthritis
Generation
Second
Vector Name
pCDCAR1
Vector Length
8kb
Vector Type
Lentiviral vector
Receptor Construction
scFv-CD28-CD3ζ
Discription of Signaling Cassetes
CD28
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatorysignals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells(APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
Antibody Clone
CIIC1; M2139
Antibody Host
Mouse

Target


Gene Name
type II collagen
Official Symbol
C2
Synonyms
C2; type II collagen; autoimmune arthritis; arthritis; scFv; FcεRIγ; Chimeric antigen receptor; T cell; Retrovirus; CARs; Lentivirus; chimeric T cell receptors; chimeric immunoreceptors; chimeric antigen receptors; CIIC1; M2139;CIIC1; M2139

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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Autoimmune Diseases

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Tel: 1-631-871-5806
Fax: 1-631-207-8356
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