pCDCAR1 ErbB2 h(4ζ), NK(CAR-NK-2-M331-4Z)

The vector of anti-ErbB2 (HER2) chimeric antigen receptor(CAR) is constructed for the engineering of NK cell to target human ErbB2. The NK cell is genetically modified through transduction with a lentiviral vector expressing scFv of anti-ErbB2 antibody linked to CD4 transmembrane domain and CD3-zeta signaling domains. And the vector product was designed for the treatment of breast carcinomas, ovarian carcinoma .


Details


Target
ErbB2 (HER2)
Target Species
Human
Targeting Cell Type
NK cell
Targeting Diseases
Breast carcinomas; ovarian carcinoma
Generation
Second
Vector Name
pCDCAR1
Vector Length
8kb
Vector Type
Lentiviral vector
Receptor Construction
scFv-CD4-CD3ζ
Discription of Signaling Cassetes
CD4
Cluster of Differentiation 4 (CD4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). CD4 signaling in CAR ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
Antibody Clone
FRP5
Antibody Host
Rabbit

Target


Gene Name
erb-b2 receptor tyrosine kinase 2
Official Symbol
ErbB2
Gene ID
Synonyms
ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; TCR; T cell receptor; transgenic TCR; antigen-specific T cell receptors; Engineering T-cell receptors; recombinant t cell receptors; specific t cell receptor; modified t cell receptor; retroviral vector; Breast cancer; ovary cancer; stomach cancer; kidney cancer; colon cancer; lung cancer

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806
Fax: 1-631-207-8356
Email:

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Tel: 44-207-048-3343

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