CellRapeutics™ Proliferation Test

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Our CAR-T cell proliferation service provides a comprehensive solution for evaluating CAR-T cell proliferation accurately and efficiently. Our commitment to scientific excellence and client satisfaction ensures that your research projects receive the support they need to succeed.

Our Services

CAR-T cell proliferation is a key factor in cancer treatment. At Creative Biolabs, our team of scientists has extensive experience in cell biology and immunology research and is able to provide customers with comprehensive and reliable proliferation assay services to support their CAR-T research projects. We provide a wide range of cell proliferation assays including MTT, MTS, CCK-8, carboxyfluorescein succinimidyl ester (CFSE), [3H] thymidine incorporation, and other proliferation assay techniques to evaluate CAR-T cell proliferation. Each assay has specific advantages and we can tailor our assays to meet specific research. Additionally, we also offer cytokine concentration assay service to measure the level of cytokines in the cell culture supernatant, which can be used as an indicator of cell activation and proliferation.

Table 1. CAR-T cell proliferation assays.

Assay	Detection Method
Colorimetric assays	MTT assay, MTS assay, XTT assay, CCK8 assay, etc.
CFSE	ICC, FACS
[3H] thymidine incorporation	FACS
EdU Assay	ICC, IHC, FACS, ELISA
Trypan Blue	Cell counting

Our Advantages

Multiple assays available: We provide various cell proliferation assays to meet the specific needs of each research project.
Fast turnaround time: Our experts have extensive experience to ensure that you can get your results on time.
Accurate data interpretation and reliable results: Our team of scientists is available to provide customers with expert advice, detailed support, and reliable results on their research projects.

If you are interested in our cell proliferation assay services, please get in touch with us for more details and learn how we support your research project. Our experts are always available to provide support, assist with your projects, and ensure your success.

Support Data

Here are some representative data for CAR-T cell proliferation assays you can refer to:

Case 1: Analysis of CAR-T cell proliferation by CCK8.

Fig.1 EGFRvIII-CAR-T cell proliferation.¹

Case 2: Analysis of CAR-T cell proliferation by CFSE.

Fig.2 CD19-CAR-T cell proliferation after stimulation with IL-2 or CD19-AVs.²

Case 3: Analysis of CAR-T cell proliferation by [3H] thymidine incorporation.

Fig.3 The proliferative capacity of HER2 CAR and IL-13Rα2 CAR T cells.³

Case 4: Analysis of CAR-T cell proliferation by trypan blue staining.

Fig.4 CAR T cells proliferation over time after co-culture with different target cells.⁴

References

Ma, Yuan, et al. "EGFRvIII-specific CAR-T cells produced by piggyBac transposon exhibit efficient growth suppression against hepatocellular carcinoma." International Journal of Medical Sciences 17.10 (2020): 1406.
Ukrainskaya, Valeria, et al. "Antigen‐Specific Stimulation and Expansion of CAR‐T Cells Using Membrane Vesicles as Target Cell Surrogates." Small 17.45 (2021): 2102643.
Hegde, Meenakshi, et al. "Combinational targeting offsets antigen escape and enhances effector functions of adoptively transferred T cells in glioblastoma." Molecular Therapy 21.11 (2013): 2087-2101.
Yazdanifar, Mahboubeh, et al. "Overcoming immunological resistance enhances the efficacy of a novel anti-tMUC1-CAR T cell treatment against pancreatic ductal adenocarcinoma." Cells 8.9 (2019): 1070.