Angiostatin

 Cat. No.

CRP0840

 Product Overview

Recombinant Human Angiostatin produced in Pichia has a molecular mass of 30498.6Da.

 Description

Angiostatin is a ~30 kDa fragment of plasminogen that has been shown to act as a potent inhibitor of angiogenesis and tumor growth in vitro and in vivo. Recombinant angiostatin is expressed in E. coli.

 Purity

≥95% as determined by  SDS-PAGE and HPLC

 Component

Angiostatin K 1-3 1mg/mL, NaAc 20 mM, NaCl 150 mM, pH 5.5.

 Specific Activity

0.55×105 Units/mg by Anti-migration Assay.The activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo.

 PI

7.8 by Isoelectricfocusing

 concentration

1.1 mg / ml by BCA

 Endotoxin

less than 10EU/mg as determined by LAL method

 Storage Buffer  

PBS Buffer.

 Storage

At 4℃ for 6 months and -80℃ for 2 years

 Gene Information

Gene Name

PLG

background

Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase  and tissue plasminogen activator. The main function of plasmin is to dissolve fibrin (see, e.g., FGA, MIM 134820) clots. Plasmin, like trypsin, belongs to the family of serine proteinases. 

Synonyms

RP1-81D8.1; DKFZp779M0222;  EC 3.4.21.7; plasminogen, Plasminogen precursor  

GeneID

5340

mRNA Refseq

NM_000301

Protein Refseq

NP_000292

MIM

173350

UniProt ID

P00747

Chromosome Location

6q26

Pathway

Complement and coagulation cascades; Neuroactive ligand-receptor interaction; Hemostasis

Function

apolipoprotein binding; calcium ion binding; peptidase activity.

process

blood coagulation, induction of apoptosis, negative regulation of angiogenesis, tissue remodeling.