BD-1

 Cat. No.

CRP0866

 Product Overview

Recombinant Human beta-Defensin 1 produced in E. coli is a single non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of approximately 5.0 KDa,.

 Description

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.

 Purity

>98% by SDS-PAGE and HPLC analyses.

 Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 130mM NaCl.   

 Biological Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1.0 ug/ml.

 Endotoxin

Less than 1EU/mg of rHuBD-1 as determined by LAL method.

 Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

 Storage

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

 Gene Information

Gene Name

DEFB1

Synonyms

BD1; DEFB-1; DEFB101; HBD1; hBD-1; beta-defensin-1; defensin beta 1; MGC51822; Beta-defensin 1 precursor; BD-1

GeneID

1672

mRNA Refseq

NM_005218.3 

Protein Refseq

NP_005209.1

MIM

602056

Unitprot ID

P60022

Chromosome Location

8p23.2-p23.1

Process

G-protein coupled receptor protein signaling pathway, chemotaxis, defense response to bacterium, innate immune response.