BD-2

CRP0867

Recombinant Human beta-Defensin 2 produced in E. coli is a single non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of  4.3 kDa.

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.

>98% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 130mM NaCl.

Fully biologically active when compared to standard. Determined by its ability to chemoattract immature human dendritic cells using a concentration range of 10-100 ng/ml.

Less than 1EU/mg of rHuBD-2 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

DEFB4

Synonyms

DEFB-2; DEFB102; DEFB2; HBD-2; SAP1; defensin; beta 4; defensin, beta 2; skin-antimicrobial peptide 1; Beta-defensin 2 precursor; Defensin β2; H.sapiens mRNA for skin-antimicrobial-peptide 1 (SAP1); defensin β4

GeneID

1673

mRNA Refseq

NM_004942.2

Protein Refseq

NP_004933.1

MIM

602215

Unitprot ID

O15263

Chromosome Location

8p23.1-p22

Process

G-protein  coupled receptor protein signaling pathway; chemotaxis; defense response to bacterium; immune response.