BMP-2

Recombinant Human Bone Morphogenetic protein-2 produced in E. coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26.018 kDa.

Bone morphogenetic protein 2 (BMP-2) is a protein that belongs to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the Hedgehog pathway, TGF-beta signaling pathway, and the Cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition.

Lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.

BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml, corresponding to a specific activity of 2.0 x 10⁴ IU/mg.

Less than 0.1ng/ug (1IEU/ug) of Bone Morphogenetic protein-2 .

It is recommended to reconstitute the lyophilized rHuBMP2 in sterile 20mM Acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Lyophilized BMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution BMP2 should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

BMP2A; BMP-2; BMP-2A; Bone morphogenetic protein 2 precursor

BMP signaling pathway; Basal cell carcinoma; Cytokine-cytokine receptor interaction; Hedgehog signaling pathway; TGF-beta signaling pathway

Cytokine activity, Growth factor activity, Specific transcriptional repressor activity