BMP-4

CRP0880

Recombinant Human Bone Morphogenetic Protein-4 produced in E. coli is a a monomeric, non-glycosylated polypeptide chain containing 116 amino acids and having a molecular mass of 13.0 kDa.

Human BMP-4 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth  factor β (TGF-β) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they havesince been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. BMP-4 is synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.

>95% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 20mM Na2CO3 buffer, pH 9.0.

Less than 1EU/μg of rHuBMP-4 as determined by LAL method. 

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

BMP4

Gene Synonyms

ZYME; BMP2B; BMP2B1; DVR4; MCOPS6; BMP-2B; BMP-4; Bone morphogenetic protein 4 precursor; bone morphogenetic protein 2B; bone morphogenetic protein 4

GeneID

652

mRNA Refseq

NM_001202

Protein Refseq

NP_001193

MIM

112262

Unitprot ID

P12644

Chromosome Location

14q22-q23

Function

cytokine activity. growth factor activity. heparin binding. protein binding. signal transducer activity.