BMP-7

CRP0890

Recombinant Human Bone Morphogenetic protein-7 produced in E. coli is a monomeric, non-glycosylated Polypeptide chain containing 139 amino acids and having a molecular mass of 15.6kDa.

Human BMP-7 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth  factor β (TGF-β) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. BMP-7 is synthesized as large precursor molecules which are cleaved by proteolytic enzymes.The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.

>95% by SDS-PAGE and HPLC analyses.

Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.

Less than 1EU/μg of rHuBMP-7 as determined by LAL method.

1. Molecular standard (Western, ELISA) in studying secreted BMP-7;

2. Preparing antibodies for BMP-7 monomer;

3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Gene Name

BMP7

Synonyms

OP-1;OP1; Osteogenic protein 1; OTTHUMP00000031357; Bone morphogenetic protein 7 precursor; Eptotermin alfa    

GeneID

655

mRNA Refseq

NM_001719

Protein Refseq

NP_001710

MIM

112267

UniProt ID

P18075

Chromosome Location

20q13

Pathway

Cytokine-cytokine receptor interaction; Hedgehog signaling pathway;TGF-beta signaling pathway

Function

cytokine activity; growth factor activity; protein binding