BNP

CRP0874

Recombinant Human Brain Natriuretic Peptide produced in E.coli is a single non-glycosylated polypeptide chain containing 32 amino acids and having a molecular mass of approximately 3.464KDa.

As a protein secreted by brain and heart artia, human brain natriuretic protein is mainly stored in cardiac ventricular myocardium. It can cause natriuresis, diuresis, vasodilation, and inhibits secretion of renin and aldosterone. It can also improves heart function.

>96% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in 0.5M NaCl, 20mM PB, pH 7.4.

Fully biologically active when compared to standard.

Less than 1EU/mg of rHuBNP as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

NPPB

Synonyms

BNP; OTTHUMP00000002506; OTTHUMP00000044486; brain type natriuretic peptide; natriuretic protein; Natriuretic peptides B precursor; brain type natriuretic peptide; natriuretic peptide precursor B; natriuretic protein

GeneID

4879

mRNA Refseq

NM_002521.2

Protein Refseq

NP_002512.1

MIM

600295

Unitprot ID

P16860

Chromosome Location

1p36.2

Function

diuretic hormone activity, hormone activity.