BLys

 Cat. No.

CRP0873

 Product Overview

Recombinant Human B Cell Activating Factor produced in E.coli is a single non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of approximately 17KDa.

 Description

B-cell activating factor (BlyS), also known as BAFF, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF,promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) andBAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF R selectively binds BAFF.

 Purity

>95% by SDS-PAGE and HPLC analyses.

 Formulation

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.0.

 Biological Activity

Fully biologically active when compared to standard. The activity is determined by the dose-dependant stimulation of IL-8 production by human PBMC. The ED50 for this effect is less than 10ng/ml.   

 Endotoxin

1EU/Less thang of rHuBLys as determined by LAL method.

 Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

 Storage

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

 Gene Information

Gene Name

TNFSF13B

Synonyms

BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; ZTNF4; ApoL related ligand TALL-1; B lymphocyte stimulator; CD257 antigen; Dendritic cell- derived TNF-like molecule; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor-like protein ZTNF4; ApoL related ligand TALL-1; B cell-activating factor; B-cell activating factor; B-lymphocyte stimulator; TNF homolog that activates apoptosis; Tumor necrosis factor ligand superfamily member 13B; delta BAFF; dendritic cell-derived TNF-like molecule; tumor necrosis factor (ligand) superfamily; member 13b; tumor necrosis factor (ligand) superfamily; member 20

GeneID

10673

mRNA Refseq

NM_006573.3

Protein Refseq

NP_006564.1

MIM

603969

Unitprot ID

Q9Y275

Chromosome Location

13q32-q34

Pathway

Cytokine-cytokine receptor interaction

Function

cytokine activity, tumor necrosis factor receptor binding.