| Description |
Cdc34 is important in the control
of cell cycle and DNA replication. Cdc34 in association with
different E3 complexes, including SCF, has been shown to target many
different substrates for ubiquitination and degradation during cell
division, signal transduction, and development. Cdc34 substrates
that have been characterized include IκB, B-Myb, Wee1, MyoD, ICERIIγ,
ATF5, p27Xic1, and p27Kip1. Additionally, substrates such as β-catenin,
p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of
Cdc34 by virtue of their SCF requirement for proteolysis. Cdc34 has
been demonstrated to self-associate through a domain in the
C-terminus, and is phosphorylated and ubiquitinylated in vivo. This
protein is useful for in vitro ubiquitinylation reactions. |
| Storage |
This
lyophilized preparation is stable for several weeks at 2-8°C, but
should be kept at -20°C for long term storage, preferably
desiccated. Upon reconstitution, the preparation is stable for up to
one week at 2-8°C. For maximal stability, apportion the
reconstituted preparation into working aliquots and store at -20°C
to -70°C. Avoid repeated freeze/thaw cycles. |
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