| Cat. No. |
CRP0896 |
| Product Overview |
Recombinant Murine
Epidermal Growth Factor produced in E. coli is a single non-glycosylated
polypeptide chain containing 53 amino acids(including 3
intramolecular disulfide-bonds) and having a molecular mass of
6.0kDa. |
| Description |
EGF was originally
discovered in crude preparations of nerve growth factor prepared
from mouse submaxillary glands as an activity that induced early
eyelid opening, incisor eruption, hair growth inhibition, and
stunting of growth when injected into newborn mice. EGF is a potent
growth factor that stimulates the proliferation of various epidermal
and epithelial cells. Additionally, EGF has been shown to inhibit
gastric secretion, and to be involved in wound healing. EGF signals
through a receptor known as c-erbB, which is a class I tyrosine
kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia
virus growth factor). |
| Purity |
>97% by SDS-PAGE and
HPLC analyses. |
| Specific Activity |
Fully biologically
active when compared to standard. The ED50 as calculated by the
dose-dependant proliferation of murine BALB/c 3T3 cells (measured by
3H-thymidine uptake) is less than 0.1 ng/ml. |
| Formulation |
Lyophilized from a
0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1EU/mg of
rmEGF as determined by LAL method. |
| Reconstitution |
We recommend that
this vial be briefly centrifuged prior to opening to bring the
contents to the bottom. Reconstitute in sterile distilled water or
aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0
mg/ml. Stock solutions should be apportioned into working aliquots
and stored at ≤20°C. Further dilutions should be made in appropriate
buffered solutions. |
| Storage |
This lyophilized
preparation is stable for several weeks at 2-8°C, but should be kept
at -20°C for long term storage, preferably desiccated. Upon
reconstitution, the preparation is stable for up to one week at
2-8°C. For maximal stability, apportion the reconstituted
preparation into working aliquots and store at -20°C to -70°C. Avoid
repeated freeze/thaw cycles. |
| Gene Information
|
Gene Name |
Egf |
|
Synonyms |
AI790464; URG; urogastrone; Pro-epidermal growth
factor precursor; epidermal growth factor; epidermal growth factor
(beta-urogastrone) |
|
GeneID |
13645 |
|
mRNA Refseq |
NM_010113 |
|
Protein Refseq |
NP_034243 |
|
MIM |
131530 |
|
UniProt ID |
Q3UWD7; P01132 |
|
Chromosome Location |
Chr 3: 129.67 - 129.75 Mb |
|
Pathway |
Cytokine-cytokine receptor interaction;
Endometrial cancer; ErbB signaling pathway; Focal adhesion; Gap
junction; Glioma; MAPK signaling pathway; Melanoma; Non-small cell
lung cancer; Pancreatic cancer; Prostate cancer; Regulation of actin
cytoskeleton |
|
Function |
calcium ion binding; growth factor activity |
|
|
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