EGF

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.

Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. EGF is a small 53 amino acid residue long protein that contains three disulfide briges.

The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 2 ng/ml, corresponding to a specific activity of 1.0 x 10⁵ IU/ mg.

Less than 0.3ng/ug (0.3IEU/ug) determined by LAL test.

It is recommended to reconstitute the lyophilized rHuEGF in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Lyophilized rHuEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuEGF should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

HOMG4, URG, Urogastrone, Pro-epidermal growth factor;Pro-epidermal growth factor precursor; epidermal growth factor (beta-urogastrone)

Cytokine-cytokine receptor interaction; Endometrial cancer;ErbB signaling pathway;Focal adhesion; Gap junction; Glioma;MAPK signaling pathway;Melanoma; Non-small cell lung cancer; Pancreatic cancer; Prostate cancer;Regulation of actin cytoskeleton; Hemostasis;Signaling by EGFR

calcium ion binding; epidermal growth factor receptor activating ligand activity; growth factor activity; protein binding