ENA-78/CXCL5

CRP08140

Recombinant Human ENA-78/CXCL5 produced in E. coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8.0 kDa.

Epithelial cell-derived neutrophil-activating peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines that has the Glu-Leu-Arg (ELR) motif preceding the CXC motif. Similar to other ELR containing CXC chemokines, ENA-78 is a potent neutrophil chemoattractant and activator. Proteolysis of ENA-78 with cathepsin G and chymotrypsin have yielded N-terminally truncated variants with increased biological activities. ENA-70 and ENA-74 represent truncated recombinant ENA-78 variants missing 8 and 4 aa residues, respectively, from the N-terminus. Recombinant ENA-70 and ENA-74 have been shown to have increased potency in neutrophil chemotaxis and myeloperoxidase and elastase release assays.

>97% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2um filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Fully biologically active when compared to standard. Determined by its ability to chemoattract human peripheral blood neutrophils  using a concentration range of 5.0-50.0 ng/ml.

Less than 1EU/mg of rHuENA-78/CXCL5 as determined by LAL method. 

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

CXCL5

Synonyms

ENA-78; SCYB5; ENA-78(1-78); ENA78; Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5; chemokine (C-X-C motif) ligand 5; epithelial-derived neutrophil activating protein 78; epithelial-derived neutrophil-activating peptide 78; neutrophil-activating protein 78; small inducible cytokine B5; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); CXCL5_HUMAN; C-X-C motif chemokine 5 [Precursor].

GeneID

6374

mRNA Refseq

NM_002994

Protein Refseq

NP_002985

MIM

600324

UniProt ID

P42830

Chromosome Location

4q12-q13

Pathway

Cytokine-cytokine receptor interaction    

Function

Chemokine activity