Endostatin

CRP0868

Recombinant Human Endostatin produced in Pichia P. is a single glycosylated polypeptide having a total molecular mass of 20 kDa.

Endostatin has been identified as a C-terminal fragment of Collagen type 18, a recently identified member of a family of collagen-like proteins referred to as multiplexin familyEndostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferation of EOMA cells. Endostatin also potently inhibits angiogenesis and tumor growth. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. Recombinant Endosatin expressed in yeast causes G1 arrest of endothelial cells, and endostatin treatment results in apoptosis of HUVE and HMVE cells.

>96% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.    

Fully biologically active when compared to standard. The activity calculated by ECE migration inhibition was found to be 50,000IU/mg.  

Less than 1EU/μg of rHuEndostatin as determined by LAL method.  

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.   

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

COL18A1

Gene Synonyms

FLJ27325, KNO, KNO1, MGC74745; OTTHUMP00000115472; OTTHUMP00000115473; alpha 1 type XVIII collagen; antiangiogenic agent; endostatin; multi-functional protein MFP; XVIII chain precursor;Collagen alpha-1; XVIIIchain precursor; collagen, type XVIII, alpha 1; human type XVIII collagen

GeneID

80781

mRNA Refseq

NM_030582.3; NM_130445.2

Protein Refseq

NP_085059.2; NP_569712.2

MIM

120328

Unitprot ID

P39060

Chromosome Location

21q22.3

Function

binding, extracellular matrix structural constituent, metal ion binding, protein binding, structural molecular activity, zinc ion binding.