Exendin-4

CRP08122

Recombinant Exendin-4 produced in E.coli is a single non-glycosylated polypeptide chain containing 39 amino acids and having a molecular mass of approximately 4.1 kDa.

Exendin-4 is a novel 39-amino acid peptide isolated from the venom of the Gila monster Heloderma suspectum. It shares 53% sequence homology with GLP-17-36amide and interacts with the same membrane receptor. Exendin-4 enhances glucose-dependent insulin secretion, suppresses inappropriately elevated glucagon secretion, and slows gastric emptying in vivo. It also promotes ss-cell proliferation and neogenesis in vitro and in animal models. Recombinant Exendin-4 is E. coli expression of a synthetic DNA squence encoding the 39 amino acid of Exendin-4.

>96% by SDS-PAGE and HPLC analyses.

141758-74-9

C184H282N50O60S

Lyophilized from a 0.2mm filtered solution of 20mM PBS, pH 7.0, containing 4% mannitol.

Sterile Filtered White lyophilized (freeze-dried) powder.

Less than 10EU/mg of rExendin-4 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.