FGF-basic

Recombinant Mouse FGF-basic produced in E. coli is a non-glycosylated polypeptide chain containing 154 amino acids and having a molecular mass of 17153 Dalton.

FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions.

Mouse FGF-basic was lyophilized from 1mg/ml solution after extensive dialysis against 20 mM phosphate buffer, pH 7.2, 50 mM Na2SO4, 0.2 mM DTT and 0.2 mM EDTA

The ED50 as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 10⁶ IU/ mg.

Less than 0.1ng/ug (1 IEU/ug) of FGF-basic.

It is recommended to reconstitute the lyophilized rmFGF-basic in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Lyophilized rmFGF-basic although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rmFGF-basic should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

DN-452A22.6, Fgf-2, Fgfb, bFGF, Prostatropin, HBGH-2, FGF-b, Heparin-binding growth factor 2, Basic fibroblast growth factor, BFGF; Heparin-binding growth factor 2 Precursor

MAPK signaling pathway; Prostate cancer; Regulation of actin cytoskeleton

growth factor activity; hepatin binding; protein binding