GDNF

CRP0845

Recombinant Human Glial Cell Line-Derived Neurotrophic Factor produced in E. coli has a molecular mass of approximately 15.2 KDa.

Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that is a member of the TGF-β superfamily. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of approximately 15 kDa. Mature rat and human GDNF share 93% sequence homology, with strong species cross-reactivity. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition,exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo. Glial cell-derived neurotrophic factor is expressed in E. coli.

>95% as determined by SDS-PAGE.

The biological activity of GDNF is measured by its ability to support survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia. Activity can also be measured by its ability to bind recombinant human GFRα1/Fc.

Liquid. (non-refolded)

Store at -80°C. Best use within three months from the date of receipt of this protein. Aliquot to avoid repeated freeze-thaw cycles.

Gene Name

GDNF

Synonyms

ATF1; ATF2; HFB1-GDNF; ATF;hGDNF; Astrocyte- derived trophic factor; Glial cell line-derived neurotrophic factor precursor; astrocyte-derived trophic factor; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial derived neurotrophic factor

GeneID

2668

mRNA Refseq

NM_000514

Protein Refseq

NP_000505

MIM

600837

Uniprot ID

P39905

Chromosome Location

5p13.1-p12

Function

growth factor activity, protein homodimerization activity

Process

anti-apoptosis, biological process, negative regulation of neuron apoptosis, nervous system development, neural development, signal transduction, regulation of dopamine uptake