GMF-β

CRP0869

Recombinant Human Glia Maturation Factor beta produced in E.coli is a single non-glycosylated polypeptide chain containing 141 amino acids and having a molecular mass of approximately 16.5KDa.

GMF-β, a brain-specific protein that belongs to the actin-binding proteins (ADF) structural family. GMF-β appears to play a role in the differentiation, maintenance, and regeneration of the nervous system. It also supports the progression of certain auto-immune diseases, possibly through its ability to induce the production and secretion of various pro-inflammatory cytokines.

>98% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 130mM NaCl.   

The biological activity was determined by the does-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <0.5ng/ml.  

rHuGMF-β as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered  solutions.  

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one  week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working  aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

GMFB

Synonyms

GMF; GMF-beta; Glia maturation factor beta; glia maturation factor; beta

GeneID

2764   

mRNA Refseq

NM_004124.2

Protein Refseq

NP_004115.1

MIM

601713

Unitprot ID

P60983

Chromosome Location

14q22.2

Function

actin binding, enzyme activator activity, growth factor activity, protein kinase inhibitor activity, signal transducer activity.