HCC-4/CCL16

CRP08111

Recombinant Human HCC-4/CCL16 produced in E. coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 11.2 kDa.

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10.

>97% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.

Fully biologically active when compared to standard. Determined by its ability to chemoattract total human monocytes using a concentration range of 10.0 -100.0 ng/ml.

Less than 1EU/mg of rHuHCC-4/CCL16 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

CCL16

Synonyms

LEC; LMC; NCC4; CKb12; HCC-4; LCC-1; Mtn-1; NCC-4; SCYL4; ILINCK; SCYA16; MGC117051; CCL16_HUMAN; C-C motif chemokine 16 [Precursor]; Small-inducible cytokine A16;IL-10-inducible chemokine;Chemokine LEC;Liver-expressed chemokine;Monotactin-1;Chemokine CC-4;Lymphocyte and monocyte chemoattractant.

GeneID

6360

mRNA Refseq

NM_004590

Protein Refseq

NP_004581

MIM

601394

UniProt ID

O15467

Chromosome Location

17q11.2

Pathway

Cytokine-cytokine receptor interaction.

Function

chemoattractant activity;chemokine activity.