I-TAC/CXCL11

CRP08113

Recombinant Human I-TAC/CXCL11 produced in E. coli is a single, non-glycosylated polypeptide chain containing 73 amino acids and having a molecular mass of 8.3 kDa.

CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence,which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-γ  and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T-cells, neutrophils or monocytes.

>97% by SDS-PAGE and HPLC analyses.

Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized from a 0.2μm filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.

Fully biologically active when compared to standard. Determined by its ability to chemoattract human IL-2 activated T-Lymphocytes using a concentration range of 0.1-10.0 ng/ml.

Less than 1EU/μg of rHuI-TAC/CXCL11 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation isstable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

CXCL11

Synonyms

IP9; Beta-R1; H174; IP-9; b-R1; ITAC;I-TAC; SCYB11; SCYB9B; MGC102770; C-X-C motif chemokine 11 precursor;Human beta-R1 mRNA, partial cds; CXL11_HUMAN; chemokine (C-X-C motif) ligand 11;Interferon-gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; Small-inducible cytokine B11; chemokine (C-X-C motif) ligand 11; small inducible cytokine B11; small inducible cytokine subfamily B (Cys-X-Cys), member 11; small inducible cytokine subfamily B (Cys-X-Cys), member.

GeneID

6373

mRNA Refseq

NM_005409

Protein Refseq

NP_005400

MIM

604852

UniProt ID

O14625

Chromosome Location

4q21.2

Pathway

Cytokine-cytokine receptor interaction;Toll-like receptor signaling pathway.

Function

chemokine activity