IFN-α1b

CRP0888

Recombinant Human Interferon-α1b produced in E.coli is a single non-glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of approximately 19 kDa.

At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

>96% by SDS-PAGE and HPLC analyses.

Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.

Fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay was found to be no less than 1.0 x 108 IU/mg.

Less than 1EU/μg of rHuIFN-α1b as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

IFNA1

Synonyms

interferon, alpha 1; IFNA@, IFL, IFN, IFN-ALPHA, IFNA13; IFN-alpha 1b, interferon alpha 1b;IFNA1_HUMAN; Interferon alpha-1/13 [Precursor]; Interferon alpha-D; LeIF D; MGC138207; MGC138505; MGC138507; OTTHUMP00000045110.

GeneID

3439

mRNA Refseq

NM_024013

Protein Refseq

NP_076918

MIM

147660

UniProt ID

P01562

Chromosome Location

9p22

Pathway

Antigen processing and presentation; Cytokine-cytokine receptor interaction;Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Regulation of autophagy; Toll-like receptor signaling pathway.