| Cat. No. |
CRP08103 |
| Product Overview |
Recombinant Ovine
Interferon-tau produced in Pichia. P is a single non-glycosylated
polypeptide chain containing 172 amino acids and having a molecular
mass of approximately 20 kDa, |
| Description |
IFN-tau is a new
class of type I IFN that is secreted by the trophoblast and is the
signal for maternal recognition of pregnancy in sheep. IFN- tau has
potent immunosuppressive and antiviral activities similar to other
type I IFN but isless cytotoxic than IFN-α/β. The current
investigation concerns the effect of recombinant ovine IFN- tau (rOvIFN-tau)
on the modulation of MHC class I and II expression on cloned mouse
cerebrovascular endothelial (CVE) cells.IFN- tau induced tyrosine
phosphorylation of Stat1 and upregulated the expression of MHC class
I on CVE. Oneproposed action by which type I IFN reduce the relapse
rate in MS is via interference with IFN-γ-induced MHC class II
expression. IFN- tau was shown to downregulate IFN-γ-induced MHC
class II expression on CVE and,hence, may be of potential
therapeutic value in downregulating inflammation in the central
nervous system (CNS). IFN- tau did not upregulate the expression of
MHC class II on CVE. IFN- tau also inhibited the replication of
Theiler's virus in CVE. |
| Purity |
>95% by SDS-PAGE and HPLC
analyses. |
| Formulation |
Lyophilized from a
0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Specific Activity |
Fully biologically
active when compared to IFN-alpha. The specific activity as
determined in a viral resistance assay was found to be no less than
1.0 x 107 IU/mg. |
| Endotoxin |
Less than 1EU/μg of rOvIFN-tau as
determined by LAL method. |
| Reconstitution |
We
recommend that this vial be briefly centrifuged prior to opening to
bring the contents to the bottom. Reconstitute in sterile distilled
water or aqueous buffer containing 0.1% BSA to a concentration of
0.1-1.0 mg/ml. Stock solutions should be apportioned into working
aliquots and stored at <-20°C. Further dilutions should be made in
appropriate buffered solutions. |
| Storage |
This
lyophilized preparation is stable for several weeks at 2-8°C, but
should be kept at -20°C for long term storage, preferably
desiccated. Upon reconstitution, the preparation is stable for up to
one week at 2-8°C. For maximal stability, apportion the
reconstituted preparation into working aliquots and store at -20°C
to -70°C. Avoid repeated freeze/thaw cycles. |
| Gene Information |
Gene Name |
IFN-tau |
|
Synonyms |
Interferon-tau;TP-1;
TP-1 trophoblast protein-1 ;
Trophoblastin; Antiluteolysin; Trophoblast antiluteolytic
protein; OTP; IFNT1; IFNT4; Interferon tau-1; Interferon tau-4 |
|
GeneID |
100144750 |
|
mRNA Refseq |
NM_001123399 |
|
Protein Refseq |
NP_001116871 |
|
UniProt ID |
P56828 |
|
|
Download Datasheet
 |
|
|
|
