|
Cat. No. |
CRP0813 |
| Product Overview |
Recombinant Human Interferon-alpha 2b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19400 Dalton. The Interferon-alpha 2b gene was obtained from human leukocytes. |
| Description |
At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. |
| Purity |
>95% as determined by SEC-HPLC and SDS-PAGE . |
| Formulation |
Lyophilized from a (1mg/ml) solution in containing 8.8mg sodium chloride, 0.4mg sodium acetate and 1.0mg acetate acid buffer. |
| Specific Activity |
The specific activity as determined in a viral resistance assay using VSV- WISH cells was found to be greater than 1.0 x 108 IU/ mg. |
| Endotoxin |
Less than 0.3ng/ug (0.3IEU/ug) determined by LAL test. |
| Reconstitution |
It is recommended to reconstitute the lyophilized rHuIFN-alpha2b in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Storage |
Lyophilized rHuIFN-alpha 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution rHuIFN-alpha 2b should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles. |
| Gene Information |
Gene Name |
IFNA2 |
|
Synonyms |
IFNA, INFA2, MGC125764, MGC125765; Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-α 2a, Interferon alpha-A, LeIF A, Interferon alpha-2; Interferon alpha-2 Precursor |
|
GeneID |
3440 |
|
mRNA Refseq |
NM_000605.2 |
|
Protein Refseq |
NP_000596.2 |
|
MIM |
147562 |
|
UniProt ID |
P01563 |
|
Chromosome Location |
9p22 |
| Pathway |
Antigen processing and presentation; Cytokine-cytokine receptor interaction ;Jak-STAT signaling pathway ;KEGG pathway: Natural killer cell mediated cytotoxicity ;Regulation of autophagy ;Toll-like receptor signaling pathway |
| Function |
interferon-alpha/beta receptor binding |
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