IFN-γ (Rat)

CRP0898

Recombinant Rat Interferon-γ produced in E. coli is a single non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15.6 kDa.

Interferon-gamma (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation.

>95% by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay is less than 0.1 ng/ml, corresponding to a specific activity of 1.0×10⁷ IU/ mg.

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.

Less than 1EU/mg of rrIFN-γ as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

Ifng

Synonyms

IFNG2; Ifg; IFN-g; IFN-gamma; Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; gamma interferon

GeneID

25712

mRNA Refseq

NM_138880

Protein Refseq

NP_620235

MIM

147570

UniProt ID

P01581

Chromosome Location

7q22

Pathway

Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Regulation of autophagy; T cell receptor signaling pathway; TGF-beta signaling pathway; Type I diabetes mellitus

Function

cytokine activity; interferon-gamma receptor binding