IL-1 alpha

CRP0878

Recombinant Human Interleukin-1 alpha produced in E.coli is a single non-glycosylated polypeptide chain containing 159 amino acids and having a molecular mass of approximately 18 KDa.

IL-1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.

>97% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.5.

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine D10S cells is less then 0.001 ng/ml, corresponding to a Specific Activity of 1 x 109 IU/mg.

Less than 1EU/mg of rHuIL-1α as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

IL1A

Synonyms

IL-1A; IL1; IL1-ALPHA; IL1F1; IL1A (IL1F1); hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha; IL-1 alpha; IL-1α; IL1A (IL1F1); Interleukin-1 alpha precursor; interleukin 1 alpha

GeneID

3552

mRNA Refseq

NM_000575.3

Protein Refseq

NP_000566.3

MIM

147760

Unitprot ID

P01583   

Chromosome Location

2q14

Pathway

Apoptosis，Cytokine-cytokine receptor interaction，Hematopoietic cell lineage，MAPK signaling pathway，Type I diabetes mellitus.

Function

interleukin-1 receptor binding, protein binding, signal transducer activity.