IL-12

CRP08147

Approximately75 kDa, a single non-glycosylated polypeptide chain containing 506 amino acids.

Inerleukin-12 (IL-12), as a potent factor, plays an important role in the networks of immune system. It is a multifunctional cytokine, the properties of which bridge innate and adaptive immunity, acting as a key regulator of cell-mediated immune responses through the induction of T helper 1differentiation. By promoting IFN-γ production, proliferation, and cytolytic activity of natural killer and T cells, IL-12 induces cellular immunity. IL-12 not only can facilitate the maturation of antigen presenting cells (APC), especially for dendritic cells (DC), but also can be the third signal to change properties of CD8 T cells and increase generation of effector and memory cells. Treatment with IL-12 can inhibit bacterial, virus and intracellular parasites, produce an effect of anti-tumor, and enhance autoimmunity. RhIL-12 is a heterodimeric cytokine composed of covalently disulfide linked a light chain of p35 (P35, IL-12α) and a heavy chain of p40 (P40, IL-12β) with PI 4.5-5.5. Specifically, the heavy chain (P40) is consisted of 306 amino acids, while the light chain (P35) compounds 197 amino acids.

Greater than 98% by SDS-PAGE gel and HPLC analyses.

10μg/bottle. Any other specifications, please feel free to contact us.

Sterile filtered through 0.2 micron filter, added the human serum albumin and mannitol and then lyophilized from 10mMPBS, pH=7.2.

the ED50 was determined by the stimulation of IFN-γ production by human peripheral blood mononuclear cells co-stimulated with IL-12 is ≤ 5ng/ml, corresponding to a specific activity of ≥ 8000 units/μg.

Endotoxin level is less than 1 EU/μg.

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 day or -20℃ for future use.

The lyophilized is stable for 3 months at room temperature, can be stored for 2 years at 4℃, but best stored at -20℃. Reconstituted IL-12 should be stored in working aliquots at -20℃.

Gene Name

IL12A

Synonyms

interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); P35; CLMF; NFSK; NKSF1; IL-12A; natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12 p35;IL-12 subunit p35;NF cell stimulatory factor chain 1; interleukin-12 alpha chain; IL12A_HUMAN; Interleukin-12 subunit alpha [Precursor];IL-12 subunit p35;Cytotoxic lymphocyte maturation factor 35 kDa subunit;CLMF p35.

GeneID

3592

mRNA Refseq

NM_000882

Protein Refseq

NP_000873

MIM

161560

UniProt ID

P29459

Chromosome Location

3q25.33-q26

Gene Name

IL12B

Synonyms

CLMF; CLMF2; IL-12B; NKSF; NKSF2; OTTHUMP00000160820; CLMF p40;Cytotoxic lymphocyte maturation factor 40 kDa subunit ; IL-12 subunit p40;IL12, subunit p40; NK cell stimulatory factor chain 2 ; cytotoxic lymphocyte maturation factor 2, p40 ; interleukin 12, p40 ; interleukin 12B ;interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic; lymphocyte maturation factor 2, p40) ; interleukin-12 beta chain ;natural killer cell stimulatory factor, 40 kD subunit ; natural killer cell stimulatory factor-2.

GeneID

3593

mRNA Refseq

NM_002187

Protein Refseq

NP_002178

UniProt ID

P29460

MIM

161561

Chromosome Location

5q31.1-q33.1

Pathway

Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus.

Function

Contributes to cytokine activity; interleukin-12 beta subunit binding;  interleukin-12 receptor binding;  interleukin-27 binding; protein binding;  protein heterodimerization activity.