IL-13

CRP0887

Recombinant Human Interleukin-13 produced in E.coli is a single non-glycosylated polypeptide chain containing 112 amino acids and having a molecular mass of approximately 12 kDa.

The human IL-13 cDNA encodes a 132 amino acid protein containing a proposed 20 amino acid signal peptide. Human IL-13 shares approximately 30% amino acid sequence homology to human IL-4 and the two cytokines exhibit overlapping biological activities. Human IL-13 is produced by activated Th0, Th1-like Th2-like and CD8 T cells. Similarly to IL-4, IL-13 has multiple effects on the differentiation and functions of monocytes/macrophages. IL-13 can suppress the cytotoxic functions of monocytes/macrophages. It can also suppress the production of proinflammatory cytokines and upregulate the production of IL-1ra by monocytes/macrophages.

>95% by SDS-PAGE and HPLC analyses

Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.2.

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of TF-1 cells was < 1.0 ng/ml, corresponding to a specific activity of > 1×106 units/mg.

Less than 1EU/mg of rHuIL-13 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

IL13

Synonyms

ALRH; BHR1; P600; IL-13; MGC116786; MGC116788; MGC116789; interleukin 13; IL13_HUMAN; Interleukin-13 [Precursor]; NC30.

GeneID

3596

mRNA Refseq

NM_002188

Protein Refseq

NP_002179

MIM

147683   

UniProt ID

P35225

Chromosome Location

5q31

Pathway

Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Jak-STAT signaling pathway.

Function

interleukin-13 receptor binding;protein binding;signal transducer activity