IL-15

Recombinant Human IL-15 produced in E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12774 Dalton.

Interleukin-15 (IL-15) is a cytokine that possesses a variety of biological functions, including stimulation and maintenance of cellular immune responses. IL-15 stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R.

The protein was lyophilized from a concentrated (1mg/ml) solution with 5mM Tris pH 8.0.

The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 10⁵ IU/ mg.

Less than 0.1 ng/ug (IEU/ug) of rHuIL-15.

It is recommended to reconstitute the lyophilized rHuIL-15 in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Lyophilized rHuIL-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuIL-15 should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway

Hematopoietin/interferon-class; cytokine receptor binding; protein binding; signal transducer activity