| Cat. No. |
CRP08112 |
| Product Overview |
Recombinant Human
IP-10/CXCL10 produced in E. coli is a single, non-glycosylated
polypeptide chain containing 77 amino acids and having a molecular
mass of 8.5 kDa. |
| Description |
IP-10 was originally
identified as an IFN-γ-inducible gene in monocytes, fibroblasts and
endothelial cells. It has since been shown that IP-10 mRNA is also
induced by LPS, IL-1β, TNF-α, IL-12 and viruses. Additional cell
typesthat have been shown to express IP-10 include activated
T-lymphocytes, splenocytes, keratinocytes, osteoblasts,astrocytes,
and smooth muscle cells. IP-10 is also expressed in psoriatic and
lepromatous lesions of skin. The mousehomologue of human IP-10,
Crg-2, has been cloned andshown to share approximately 67% amino
acid sequenceidentity with human IP-10. |
| Purity |
>97% by SDS-PAGE and
HPLC analyses. |
| Formulation |
Lyophilized from a
0.2μm filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4,
50mM NaCl |
| Specific Activity |
Fully biologically
active when compared to standard. Determined by its ability to
chemoattract human T-Lymphocytes using a concentration range of
10.0-100.0 ng/ml. |
| Endotoxin |
Less than 1EU/μg of
rHuIP-10/CXCL10 as determined by LAL method. |
| Reconstitution |
We
recommend that this vial be briefly centrifuged prior to opening to
bring the contents to the bottom. Reconstitute in sterile distilled
water or aqueous buffer containing 0.1% BSA to a concentration of
0.1-1.0 mg/ml. Stock solutions should be apportioned into working
aliquots and stored at <-20°C. Further dilutions should be made in
appropriate buffered solutions. |
| Storage |
This
lyophilized preparation is stable for several weeks at 2-8°C, but
should be kept at -20°C for long term storage, preferably
desiccated. Upon reconstitution, the preparation isstable for up to
one week at 2-8°C. For maximal stability, apportion the
reconstituted preparation into working aliquots and store at -20°C
to -70°C. Avoid repeated freeze/thaw cycles. |
| Gene Information |
Gene Name |
CXCL10 |
|
Synonyms |
C7;IFI10;INP10;IP-10;crg-2;
CXL10_HUMAN;mob-1;SCYB10;gIP-10;Gamma-IP10 ;10 kDa
interferon-gamma-induced protein; C-X-C motif chemokine 10
precursor; Small-inducible cytokine B10; chemokine (C-X-C motif)
ligand 10; gamma IP10; interferon (gamma)-induced cell line; protein
10 from; interferon-inducible cytokine IP-10; protein 10 from
interferon (gamma)-induced cell line; small inducible cytokine B10;
small inducible cytokine subfamily B (Cys-X-Cys), member 10.
|
|
GeneID |
3627 |
|
mRNA Refseq |
NM_001565 |
|
Protein Refseq |
NP_001556 |
|
MIM |
147310 |
|
UniProt ID |
P02778 |
|
Chromosome Location |
4q21 |
|
Pathway |
Cytokine-cytokine
receptor interaction; Toll-like receptor signaling pathway.
|
|
Function |
cAMP-dependent protein kinase regulator activity;chemokine
activity |
|
|
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