CRP0882

Recombinant Human Keratinocye Growth Factor-1 produced in E. coli is a single non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of approximately 19.0 kDa.

Keratinocyte Growth Factor-1 (KGF-1/FGF-7) is one of 23 known members of the FGF family. All FGFs have two conserved cysteine residues and share 30 - 50% sequence identity at the amino acid level. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of variety of tissues, by promoting cellular proliferation and differentiation. KGF-1/FG-7 is a mitogen factor specific for epithelial cells and keratinocytes and signals through FGFR 2b. KGF-1/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing.

>96% by SDS-PAGE and HPLC analyses.

The biological activity was determined by the does-dependent stimulation of thymidine uptake by BaF3 cells expressing KGF receptors yielding an ED50 <10ng/ml.

Less than 1EU/mg of rHuKGF-1 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.   

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

FGF7

Synonyms

KGF; HBGF-7; FGF-7; Fibroblast growth factor 7; keratinocyte growth factor; Keratinocyte growth factor precursor; fibroblast growth factor 7 (keratinocyte growth factor)

GeneID

2252

mRNA Refseq

NM_002009

Protein Refseq

NP_002000

MIM

148180

Unitprot ID

P21781

Chromosome Location

15q15-q21.1

Pathway

MAPK signaling pathway. Melanoma. Prostate cancer. Regulation of actin cytoskeleton. Signaling by FGFR  

Function

growth factor activity