KGF-2

CRP0881

Recombinant Human Keratinocye Growth Factor-2 produced in E. coli is a 170 amino acid residues consisting of Methionine and the mature human KGF-2 (amino acid residues 40–208) and having a molecular mass of approximately 19.3 kDa.

KGF-2(also known as FGF-10) was originally identified from rat embryos by homology-based polymerase chain reaction. Human and mouse KGF-2 were subsequently cloned. The human KGF-2 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human KGF-2 shares approximately 92% and  95% amino acid sequence identity with mouse and rat KGF-2, respectively. Among the FGF family members, KGF-2 is most closely related to FGF-7. The expression of KGF-2 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant KGF-2 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, KGF-2 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.

>96% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.  

The biological activity was determined by the does-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <0.5ng/ml.

Less than 1EU/μg of rHuKGF-2 as determined by LAL method.  

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered  solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

FGF10

Synonyms

Fibroblast growth factor 10 precursor; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria; fibroblast growth factor 10

GeneID

2255

mRNA Refseq

NM_004465

Protein Refseq

NP_004456

MIM

602115

Unitprot ID

O15520

Chromosome Location

5p13-p12

Pathway

MAPK signaling pathway. Melanoma. Regulation of actin cytoskeleton. Signaling by FGFR

Function

chemoattractant activity. fibroblast growth factor receptor binding. growth factor activity. heparin binding. protein binding