MIC-A

CRP08128

Recombinant Human MIC-A produced in E.coli is protein with 320 amino acid residues containing the extracellular domain of mature human MICA (amino acid residues Ala23 – Gln308) and having a molecular mass of approximately 36 kDa.

MIC-A (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with β2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells,       γδ T cells, and CD8+ αβ T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

>95% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.

Measured by its ability to bind MICA antibody in a ELISA.

Less than 1EU/μg of rHuMIC-A as determined by LAL method.

Sterile Filtered White lyophilized (freeze-dried) powder.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

HLA-A

Synonyms

major histocompatibility complex, class I, A; 1A31_HUMAN; HLA class I histocompatibility antigen, A-31; alpha chain [Precursor]; MHC class I antigen A*31; HLAA;A-1;A-10; A-28; A-9; Aw-19; Aw-24; Aw-33; Aw-34; Aw-36; Aw-43; Aw-66; Aw-68; Aw-69; Aw-74;Aw-80; HLA class I; HLA class I histocompatibility antigen, A alpha chain precursor; MHC class I antigen HLA-A heavy chain; MHC leukocyte antigen; antigen presenting molecule; leucocyte antigen class I;leukocyte antigen class I-A.

GeneID

3105

mRNA Refseq

NM_002116

Protein Refseq

NP_002107

MIM

142800

UniProt ID

P04439

Chromosome Location

6p21.3

Pathway

Antigen processing and presentation; Cell adhesion molecules (CAMs); Natural killer cell mediated cytotoxicity; Type I diabetes mellitus; Signaling in Immune system.

Function

MHC class I receptor activity; protein binding.