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Cat. No. |
CRP08129 |
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Product
Overview |
Recombinant Human MIC-B produced in
E.coli is a
protein with
326 amino acid residues containing the extracellular domain of
mature human MICB (amino acid residues Ala23 – Tyr312)
and having a molecular mass of approximately
37 kDa. |
|
Description |
MIC-B (MHC class I chain-related gene B) is a transmembrane
glycoprotein that functions as a ligand for human NKG2D. A closely
related protein, MIC-A, shares 85% amino acid identity with MIC-B.
These 2 proteins are distantly related to the MHC class I proteins.
MIC-A and MIC-B (MIC-A/B) possess three extracellular
immunoglobulin-like domains, but have no capacity to bind peptide or
interact with β2-microglobulin. The genes encoding MIC-A/B are
found within the major histocompatibility complex on human
chromosome 6. The MIC-B locus is polymorphic with more than 15
recognized human alleles. MIC-A/B are minimally expressed on normal
cells, but are frequently expressed on epithelial tumors and can be
induced by bacterial and viral infections.MIC-A/B are ligands for
NKG2D, an activating receptor expressed on NK cells, NKT
cells, γδ T cells, and CD8+ αβ T cells. Recognition of
MIC-A/B by NKG2D results in the activation of cytolytic activity
and/or cytokine production by these effector cells. MIC-A/B
recognition is involved in tumor surveillance, viral infections, and
autoimmune diseases. The release of soluble forms of MIC-A/B from
tumors down-regulates NKG2D surface expression on effector cells
resulting in the impairment of anti-tumor immune response. |
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Purity |
>95% by SDS-PAGE and HPLC analyses. |
|
Formulation |
Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in
PBS, pH 7.0 |
|
Specific Activity |
Measured by its ability to bind MICB antibody in a ELISA. |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Endotoxin |
Less than 1EU/μg of rHuMIC-B as determined by LAL method. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening
to bring the contents to the bottom. Reconstitute in sterile
distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be
apportioned into working aliquots and stored at <-20°C. Further
dilutions should be made in appropriate buffered solutions. |
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Storage |
This lyophilized preparation is stable at 2-8°C, but should be kept
at -20°C for long term storage, preferably desiccated. Upon
reconstitution, the preparation is stable for up to one week at
2-8°C. For maximal stability, apportion the reconstituted
preparation into working alire 0t -C to -70°C. Avoid
repeated freeze/thaw cycles. |
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Gene
Information
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Gene
Name |
HLA-B |
|
Synonyms |
AS; HLAB; HLAC; SPDA1; HLA-B73; HLA-B-7301; major histocompatibility
complex, class I, B; ankylosing spondylitis; 1B53_HUMAN; HLA class I
histocompatibility antigen, B-53; alpha chain [Precursor]; MHC class
I antigen; B*53; Bw-53;
HLAB;B'DT;B-12;B-21;B-5;HLA-B-5501;OTTHUMP00000034826; HLA class I
histocompatibility antigen, B alpha chain; HLA class I
histocompatibility antigen, B alpha chain precursor; Human
transforming growth factor-beta induced gene product (BIGH3);mRNA,
complete cds; MHC HLA-B cell surface glycoprotein;MHC class I
HLA-B40MD alpha chain; MHC class I HLA-C antigen; MHC class I
antigen B*13; MHC class I antigen HLA-B heavy chain; MHC class I
antigen SHCHA; ankylosing spondylitis; leucocyte antigen C;
leukocyte antigen class I-B; lymphocyte antigen; major
histocompatibility complex class I B; major histocompatibility
complex, class I, B. |
|
GeneID |
3106 |
|
mRNA
Refseq |
NM_005514 |
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Protein
Refseq |
NP_005505 |
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MIM |
142830 |
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Chromosome Location |
6p21.3 |
|
Pathway |
Antigen
processing and presentation; Cell adhesion molecules (CAMs); Natural
killer cell mediated cytotoxicity;Type I diabetes mellitus;Signaling
in Immune system. |
|
Function |
MHC
class I receptor activity;molecular_function;protein binding. |
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