MIG/CXCL9

CRP08145

Recombinant Human MIG/CXCL9 produced in E. coli is a single, non-glycosylated polypeptide chain containing 103 amino acids and having a molecular mass of 11.7 kDa.

CXCL9, a member of the α subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-γ. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid residue precursor protein with a 22 amino acid residue signal peptide that is cleaved to yield a 103 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has recently been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

>97% by SDS-PAGE and HPLC analyses.

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Fully biologically active when compared to standard. Determined by its ability to chemoattract human peripheral blood T-Lymphocytes using a concentration range of 10.0-100.0 ng/ml.

Less than 1EU/mg of rHuMIG/CXCL9 as determined by LAL method.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Gene Name

CXCL9 chemokine (C-X-C motif) ligand 9

Synonyms

CMK; MIG; Humig; SCYB9; crg-10; Gamma-interferon-induced monokine; Small-inducible cytokine B9; chemokine (C-X-C motif) ligand 9; monokine induced by gamma interferon; small inducible cytokine B9; CXCL9_HUMAN; C-X-C motif chemokine 9 [Precursor]; Gamma-interferon-induced monokine.

GeneID

4283

mRNA Refseq

NM_002416

Protein Refseq

NP_002407

MIM

601704

UniProt ID

Q07325

Chromosome Location

4q21

Pathway

Cytokine-cytokine receptor interaction;Toll-like receptor signaling pathway

Function

chemokine activity